The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.
Subcellular Location:
Secreted
Cytoplasmic granule membrane
Membrane-associated in polymorphonuclear Leukocytes (PMN) granules.
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.7
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.2
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Recovery
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Recovery was determined by spiking various levels of BPI into serum and plasma .
Sample Type
Average(%)
Recovery Range (%)
Serum
99
91-108
Plasma
95
89-97
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BPI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
80-92%
94-105%
96-103%
95-108%
EDTA plasma(n=5)
91-102%
96-103%
89-95%
86-102%
heparin plasma(n=5)
84-98%
85-99%
79-91%
96-101%
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Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 32-68;FUNCTION;SUBCELLULAR LOCATION;DOMAIN;VARIANTS VAL-16 AND LYS-216
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.
"cDNA cloning and sequence analysis of human bactericidal/permeability protein." Ma H.
,
Cao X.
,
Sun Y.
,
Lei W.
Submitted (2006-02) to the EMBL/GenBank/DDBJ databases
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT VAL-16
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT ASP-404
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT LYS-216
"Cloning of cDNA of human bactericidal/permeability-increasing protein." Xu J.
,
Wang H.
Submitted (2000-11) to the EMBL/GenBank/DDBJ databases
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-487;VARIANTS LYS-216 AND ASP-404
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: SUBUNIT;SUBCELLULAR LOCATION;MUTAGENESIS OF SER-49; CYS-163; CYS-166 AND CYS-206
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 32-487
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 32-487;DISULFIDE BOND